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CRISPR gRNA (guide RNA) Design Tool for Eukaryotic Pathogens

Please fill out the following fields: job name, sequence. One or more parameter you entered is not within range, guide RNA length should be between 4 and 50, homology arm length should be between 50 and 500, identity should be between 0 and 1 coverage should be between 0 to 1. PAM length should be between 3 and 10, and containing only letters "A", "T", "C", "G", "N", no space(s). Please paste your input sequence to search guide RNA in. Plain sequence or FASTA format. Genome selected to run the example.

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CRISPR gRNA (guide RNA) Design Tool for Eukaryotic Pathogens | grna.ctegd.uga.edu Reviews

<META> DESCRIPTION

Please fill out the following fields: job name, sequence. One or more parameter you entered is not within range, guide RNA length should be between 4 and 50, homology arm length should be between 50 and 500, identity should be between 0 and 1 coverage should be between 0 to 1. PAM length should be between 3 and 10, and containing only letters A, T, C, G, N, no space(s). Please paste your input sequence to search guide RNA in. Plain sequence or FASTA format. Genome selected to run the example.

<META> KEYWORDS

1 plain sequence example

2 fasta formate example

3 example 1

4 cl strain genome

5 example 2

6 atcgatcgatcgatcgatcg nag

7 atcgatcgatcgatcgatcg nga

8 in undesired loci

9 in job names

10 spcas9

CONTENT

Page content here

KEYWORDS ON PAGE

plain sequence example,fasta formate example,example 1,cl strain genome,example 2,atcgatcgatcgatcgatcg nag,atcgatcgatcgatcgatcg nga,in undesired loci,in job names,spcas9,sacas9,iupac nucleotide code,base,adenine,cytosine,guanine,thymine,a or g,c or t,n sp

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CRISPR gRNA (guide RNA) Design Tool for Eukaryotic Pathogens | grna.ctegd.uga.edu Reviews

https://grna.ctegd.uga.edu

Please fill out the following fields: job name, sequence. One or more parameter you entered is not within range, guide RNA length should be between 4 and 50, homology arm length should be between 50 and 500, identity should be between 0 and 1 coverage should be between 0 to 1. PAM length should be between 3 and 10, and containing only letters "A", "T", "C", "G", "N", no space(s). Please paste your input sequence to search guide RNA in. Plain sequence or FASTA format. Genome selected to run the example.

INTERNAL PAGES

grna.ctegd.uga.edu

1

T. cruzi gRNA design

http://grna.ctegd.uga.edu/HDRtemplate.html

Eukaryotic Pathogen CRISPR gRNA Design Tool. With (1) custom genome upload, (2) off-target analysis, (3) on-targets searching (for targeting gene families), (4) efficiency/activity prediction, (5) assisted oligo repair template design , (6) gRNA transcription problem identification, (7) flanking microhomology searching (for predicting deletions). Tarleton Research Group, The Center for Tropical and Emerging Global Diseases, The University of Georgia.

2

CRISPR gRNA (guide RNA), gDNA (guide DNA) Design Tool for Eukaryotic Pathogens

http://grna.ctegd.uga.edu/batch.html

Please fill out the following fields: job name, email, sequence. One or more parameter you entered is not within range, gRNA length should be between 4 and 50, homology arm length should be between 50 and 500, identity should be between 0 and 1 coverage should be between 0 to 1. PAM length should be between 3 and 10, and containing only letters "A", "T", "C", "G", "N", no space(s). Please paste your input sequence to search gRNA in. Plain sequence or FASTA format. Genome selected to run the example.

3

CRISPR gRNA (guide RNA), gDNA (guide DNA) Design Tool for Eukaryotic Pathogens

http://grna.ctegd.uga.edu/batch_tagging.html

Please fill out the following fields: job name, email, sequence. One or more parameter you entered is not within range, gRNA length should be between 4 and 50, homology arm length should be between 50 and 500, identity should be between 0 and 1 coverage should be between 0 to 1. PAM length should be between 3 and 10, and containing only letters "A", "T", "C", "G", "N", no space(s). Please paste your input sequence to search gRNA in. Plain sequence or FASTA format. Genome selected to run the example.

4

T. cruzi gRNA design

http://grna.ctegd.uga.edu/citation.html

Eukaryotic Pathogen CRISPR gRNA Design Tool. With (1) custom genome upload, (2) off-target analysis, (3) on-targets searching (for targeting gene families), (4) efficiency/activity prediction, (5) assisted oligo repair template design , (6) gRNA transcription problem identification, (7) flanking microhomology searching (for predicting deletions). Tarleton Research Group, The Center for Tropical and Emerging Global Diseases, The University of Georgia.

5

T. cruzi gRNA design

http://grna.ctegd.uga.edu/pipeline.html

Eukaryotic Pathogen CRISPR gRNA Design Tool. With (1) custom genome upload, (2) off-target analysis, (3) on-targets searching (for targeting gene families), (4) efficiency/activity prediction, (5) assisted oligo repair template design , (6) gRNA transcription problem identification, (7) flanking microhomology searching (for predicting deletions). Tarleton Research Group, The Center for Tropical and Emerging Global Diseases, The University of Georgia.

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Publications on EuPathDB sites. Submitting data to SchistoDB. How to submit data to us. EuPathDB Data Submission and Release Policies. How to cite us. Publications that Use our Resources. EuPathDB Brochure in Chinese. NCBI's Glossary of Terms. Text, IDs, Organism. Text (product name, notes, etc.). Search for Genomic Sequences. Search for Genomic Segments (DNA Motif). Extent of Gene Overlap. Search for Metabolic Pathways. Text (synonym, InChI, etc.). View all available searches. Searches via Web Services.

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Publications on EuPathDB sites. Submitting data to GiardiaDB. How to submit data to us. EuPathDB Data Submission and Release Policies. How to cite us. Publications that Use our Resources. EuPathDB Brochure in Chinese. NCBI's Glossary of Terms. Text, IDs, Organism. Text (product name, notes, etc.). Mass Spec. Evidence. Text (search product name, notes, submitter etc.). Search for Genomic Sequences. Search for Genomic Segments (DNA Motif). Extent of Gene Overlap. Search for SAGE Tags. New Data Sets page!

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Publications on EuPathDB sites. Submitting data to GiardiaDB. How to submit data to us. EuPathDB Data Submission and Release Policies. How to cite us. Publications that Use our Resources. EuPathDB Brochure in Chinese. NCBI's Glossary of Terms. Text, IDs, Organism. Text (product name, notes, etc.). Mass Spec. Evidence. Text (search product name, notes, submitter etc.). Search for Genomic Sequences. Search for Genomic Segments (DNA Motif). Extent of Gene Overlap. Search for SAGE Tags. New Data Sets page!

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Publications on EuPathDB sites. Submitting data to FungiDB. How to submit data to us. EuPathDB Data Submission and Release Policies. How to cite us. Publications that Use our Resources. EuPathDB Brochure in Chinese. NCBI's Glossary of Terms. Text, IDs, Organism. Text (product name, notes, etc.). Text (search product name, notes, submitter etc.). Search for Genomic Sequences. Search for Genomic Segments (DNA Motif). Differences Within a Group of Isolates. Differences Between Two Groups of Isolates. High T...

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http://fungidb.org/fungidb/categoryPage.jsp?record=GeneRecordClasses.GeneRecordClass&category=other_attributes

Publications on EuPathDB sites. Submitting data to FungiDB. How to submit data to us. EuPathDB Data Submission and Release Policies. How to cite us. Publications that Use our Resources. EuPathDB Brochure in Chinese. NCBI's Glossary of Terms. Text, IDs, Organism. Text (product name, notes, etc.). Text (search product name, notes, submitter etc.). Search for Genomic Sequences. Search for Genomic Segments (DNA Motif). Differences Within a Group of Isolates. Differences Between Two Groups of Isolates. Only a...

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http://fungidb.org/fungidb/app/answer/DatasetQuestions.AllDatasets

Publications on EuPathDB sites. Submitting data to FungiDB. How to submit data to us. EuPathDB Data Submission and Release Policies. How to cite us. Publications that Use our Resources. EuPathDB Brochure in Chinese. NCBI's Glossary of Terms. Text, IDs, Organism. Text (product name, notes, etc.). Text (search product name, notes, submitter etc.). Search for Genomic Sequences. Search for Genomic Segments (DNA Motif). Differences Within a Group of Isolates. Differences Between Two Groups of Isolates. Genome...

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http://fungidb.org/common/downloads

Publications on EuPathDB sites. Submitting data to FungiDB. How to submit data to us. EuPathDB Data Submission and Release Policies. How to cite us. Publications that Use our Resources. EuPathDB Brochure in Chinese. NCBI's Glossary of Terms. Text, IDs, Organism. Text (product name, notes, etc.). Text (search product name, notes, submitter etc.). Search for Genomic Sequences. Search for Genomic Segments (DNA Motif). Differences Within a Group of Isolates. Differences Between Two Groups of Isolates. If you...

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grna.ctegd.uga.edu

CRISPR gRNA (guide RNA) Design Tool for Eukaryotic Pathogens

Please fill out the following fields: job name, sequence. One or more parameter you entered is not within range, guide RNA length should be between 4 and 50, homology arm length should be between 50 and 500, identity should be between 0 and 1 coverage should be between 0 to 1. PAM length should be between 3 and 10, and containing only letters "A", "T", "C", "G", "N", no space(s). Please paste your input sequence to search guide RNA in. Plain sequence or FASTA format. Genome selected to run the example.

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