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Molecular biology protocols, tutorials…and blog! 10x Running buffer for Tris-Glycine SDS-PAGE. 4x SDS Protein Sample Buffer. Coomassie blue staining solution for polyacrylamide gels. Purification of GST-fusion proteins from E. coli. Easy single-primer site-directed mutagenesis. Are ultra low-temperature freezers and liquid nitrogen tanks really necessary? January 8, 2017. Off-patent: DNAzol from Thermo Fisher (Invitrogen). January 8, 2017. A molecular biology lab in my basement? October 17, 2015. I ran a...

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mBIOnet | Molecular biology protocols, tutorials…and blog! | mbionet.com Reviews

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Molecular biology protocols, tutorials…and blog! 10x Running buffer for Tris-Glycine SDS-PAGE. 4x SDS Protein Sample Buffer. Coomassie blue staining solution for polyacrylamide gels. Purification of GST-fusion proteins from E. coli. Easy single-primer site-directed mutagenesis. Are ultra low-temperature freezers and liquid nitrogen tanks really necessary? January 8, 2017. Off-patent: DNAzol from Thermo Fisher (Invitrogen). January 8, 2017. A molecular biology lab in my basement? October 17, 2015. I ran a...

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2 buffer recipes

3 hnmetg lysis buffer

4 mripa lysis buffer

5 plasmid sequences

6 pcdna3 1 human ub wt

7 protocols

8 directional pcr subcloning

9 tutorials

10 posted on

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mBIOnet | Molecular biology protocols, tutorials…and blog! | mbionet.com Reviews

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Molecular biology protocols, tutorials…and blog! 10x Running buffer for Tris-Glycine SDS-PAGE. 4x SDS Protein Sample Buffer. Coomassie blue staining solution for polyacrylamide gels. Purification of GST-fusion proteins from E. coli. Easy single-primer site-directed mutagenesis. Are ultra low-temperature freezers and liquid nitrogen tanks really necessary? January 8, 2017. Off-patent: DNAzol from Thermo Fisher (Invitrogen). January 8, 2017. A molecular biology lab in my basement? October 17, 2015. I ran a...

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pCDNA3.1-human-ub-wt | mBIOnet

http://mbionet.com/plasmid-sequences/pcdna3-1-human-ub-wt

Molecular biology protocols, tutorials…and blog! 10x Running buffer for Tris-Glycine SDS-PAGE. 4x SDS Protein Sample Buffer. Coomassie blue staining solution for polyacrylamide gels. Purification of GST-fusion proteins from E. coli. Easy single-primer site-directed mutagenesis. Virtual plasmid consisting of wild-type human ubiquitin cloned between KpnI and NotI sites of pCDNA3.1 -Zeo. Used for site-directed mutagenesis tutorial. 1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC. 851 GCTTATCGAA ATT...

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10x Running buffer for Tris-Glycine SDS-PAGE | mBIOnet

http://mbionet.com/15-2/10x-running-buffer-for-tris-glycine-sds-page

Molecular biology protocols, tutorials…and blog! 10x Running buffer for Tris-Glycine SDS-PAGE. 4x SDS Protein Sample Buffer. Coomassie blue staining solution for polyacrylamide gels. Purification of GST-fusion proteins from E. coli. Easy single-primer site-directed mutagenesis. 10x Running buffer for Tris-Glycine SDS-PAGE. Recipe for 2 l:. 604 g Tris base. Leave a Reply Cancel reply. You must be logged in. To post a comment. A molecular biology lab in my basement? A representative western blot….

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January | 2015 | mBIOnet

http://mbionet.com/2015/01/94

Molecular biology protocols, tutorials…and blog! 10x Running buffer for Tris-Glycine SDS-PAGE. 4x SDS Protein Sample Buffer. Coomassie blue staining solution for polyacrylamide gels. Purification of GST-fusion proteins from E. coli. Easy single-primer site-directed mutagenesis. Monthly Archives: January 2015. A representative western blot…. January 6, 2015. A molecular biology lab in my basement? A representative western blot…. Antibody validation: journals’ expectations vs. commercial suppliers.

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Uncategorized | mBIOnet

http://mbionet.com/category/uncategorized

Molecular biology protocols, tutorials…and blog! 10x Running buffer for Tris-Glycine SDS-PAGE. 4x SDS Protein Sample Buffer. Coomassie blue staining solution for polyacrylamide gels. Purification of GST-fusion proteins from E. coli. Easy single-primer site-directed mutagenesis. A molecular biology lab in my basement? October 17, 2015. What are the components of an average, modern molecular biology lab? A representative western blot…. January 6, 2015. December 21, 2014. Adventures in peer review. The issu...

5

HNMETG lysis buffer | mBIOnet

http://mbionet.com/15-2/hnmetg-lysis-buffer

Molecular biology protocols, tutorials…and blog! 10x Running buffer for Tris-Glycine SDS-PAGE. 4x SDS Protein Sample Buffer. Coomassie blue staining solution for polyacrylamide gels. Purification of GST-fusion proteins from E. coli. Easy single-primer site-directed mutagenesis. 50 mM HEPES/NaOH pH 7.5. 10 ml 1 M HEPES/NaOH. 6 ml 5 M NaCl. 300 ul 1 M MgCl2. 400 ul 0.5 M EGTA. 2 ml Triton X-100. 1613 ml H20 (to 200 ml). Leave a Reply Cancel reply. You must be logged in. To post a comment.

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mBIOnet | Molecular biology protocols, tutorials…and blog!

Molecular biology protocols, tutorials…and blog! 10x Running buffer for Tris-Glycine SDS-PAGE. 4x SDS Protein Sample Buffer. Coomassie blue staining solution for polyacrylamide gels. Purification of GST-fusion proteins from E. coli. Easy single-primer site-directed mutagenesis. Are ultra low-temperature freezers and liquid nitrogen tanks really necessary? January 8, 2017. Off-patent: DNAzol from Thermo Fisher (Invitrogen). January 8, 2017. A molecular biology lab in my basement? October 17, 2015. I ran a...

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