ragonfacs.blogspot.com

Just the FACS

Monday, December 31, 2012. I've decided to post to the New England Cytometry blog instead of here, so if what I've written about flow is helpful to you, I hope you'll follow me there! Just copying over some of the topics I've covered here so far, with new material on the horizon. Ragon Institute Flow Core. Monday, August 29, 2011. Mismatches in your positive and negative particles will result in compensation errors, which could dramatically impact your data. More on that next post. Tuesday, July 20, 2010.

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Just the FACS | ragonfacs.blogspot.com Reviews

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Monday, December 31, 2012. I've decided to post to the New England Cytometry blog instead of here, so if what I've written about flow is helpful to you, I hope you'll follow me there! Just copying over some of the topics I've covered here so far, with new material on the horizon. Ragon Institute Flow Core. Monday, August 29, 2011. Mismatches in your positive and negative particles will result in compensation errors, which could dramatically impact your data. More on that next post. Tuesday, July 20, 2010.

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Just the FACS | ragonfacs.blogspot.com Reviews

https://ragonfacs.blogspot.com

Monday, December 31, 2012. I've decided to post to the New England Cytometry blog instead of here, so if what I've written about flow is helpful to you, I hope you'll follow me there! Just copying over some of the topics I've covered here so far, with new material on the horizon. Ragon Institute Flow Core. Monday, August 29, 2011. Mismatches in your positive and negative particles will result in compensation errors, which could dramatically impact your data. More on that next post. Tuesday, July 20, 2010.

INTERNAL PAGES

ragonfacs.blogspot.com

1

Just the FACS: April 2010

http://ragonfacs.blogspot.com/2010_04_01_archive.html

Thursday, April 29, 2010. Thanks for visiting the blog of the Flow Cytometry Core Facility of the Ragon Institute of MGH, MIT, and Harvard. I plan to post different topics relating to flow cytometry, including technology, common issues, how-to's, etc. Any suggestions for topics you'd like to see please email ragonfacs@gmail.com. And click on the "Flow Cytometry" link on the right side. Ragon Institute Flow Core. Subscribe to: Posts (Atom). Ragon Institute Flow Core. View my complete profile.

2

Just the FACS: August 2011

http://ragonfacs.blogspot.com/2011_08_01_archive.html

Monday, August 29, 2011. All fluorochromes will emit light across a range of wavelenghts, and if you are trying to take measurements for another fluor in a band that is included in this range, you will get background light ("spillover" or "spectral overlap") from that other fluorochrome. To correct for this, most cytometry software has some mechanism for "compensation" to remove this background. In essence, for any other parameter/detector. Ragon Institute Flow Core. Subscribe to: Posts (Atom).

3

Just the FACS: July 2010

http://ragonfacs.blogspot.com/2010_07_01_archive.html

Tuesday, July 20, 2010. So it was the XXVth (is that even a term? Chchivith in Nahuatl) ISAC Congress, and just in time since my computer bag from the last North American congress (Quebec City in ’06, they alternate every 2 years between N.A. and Europe and I was unable to go to Budapest in ‘08) was just about worn out. I don’t know what they were thinking this year, there are 5 weekends in May and they have to pick Mothers Day weekend? Can you compost the paper cup or is it trash? I guess I can’t ...

4

Just the FACS: Compensation

http://ragonfacs.blogspot.com/2010/08/compensation.html

Monday, August 29, 2011. All fluorochromes will emit light across a range of wavelenghts, and if you are trying to take measurements for another fluor in a band that is included in this range, you will get background light ("spillover" or "spectral overlap") from that other fluorochrome. To correct for this, most cytometry software has some mechanism for "compensation" to remove this background. In essence, for any other parameter/detector. Ragon Institute Flow Core. Subscribe to: Post Comments (Atom).

5

Just the FACS: We have moved!

http://ragonfacs.blogspot.com/2012/12/we-have-moved.html

Monday, December 31, 2012. I've decided to post to the New England Cytometry blog instead of here, so if what I've written about flow is helpful to you, I hope you'll follow me there! Just copying over some of the topics I've covered here so far, with new material on the horizon. Ragon Institute Flow Core. Subscribe to: Post Comments (Atom). Ragon Institute Flow Core. View my complete profile.

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ragonfacs.blogspot.com

Just the FACS

Monday, December 31, 2012. I've decided to post to the New England Cytometry blog instead of here, so if what I've written about flow is helpful to you, I hope you'll follow me there! Just copying over some of the topics I've covered here so far, with new material on the horizon. Ragon Institute Flow Core. Monday, August 29, 2011. Mismatches in your positive and negative particles will result in compensation errors, which could dramatically impact your data. More on that next post. Tuesday, July 20, 2010.

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