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tsienlab.ucsd.edu

Tsien lab Website

Department of Chemistry and Biochemistry. Website Maintained by Paul Steinbach.

http://tsienlab.ucsd.edu/

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Tsien lab Website | tsienlab.ucsd.edu Reviews
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Department of Chemistry and Biochemistry. Website Maintained by Paul Steinbach.
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Tsien lab Website | tsienlab.ucsd.edu Reviews

https://tsienlab.ucsd.edu

Department of Chemistry and Biochemistry. Website Maintained by Paul Steinbach.

INTERNAL PAGES

tsienlab.ucsd.edu tsienlab.ucsd.edu
1

Images

http://tsienlab.ucsd.edu/Samples.htm

The Tsien lab no longer sends out samples directly. All of our constructs, listed HERE. Are available either commercially or through Addgene, to non-profit or academic institutions. If you are a commercial enterprise who would like to license our materials, please contact Rose Murphy, Senior Licensing Officer, in the Technology Transfer office of UCSD, rmmurphy@ucsd.edu. To request permission to obtain a secondary DNA sample from a 3rd party. Who is in possession of one of our constructs: Click Here.

2

Publications

http://tsienlab.ucsd.edu/Publication.htm

Jorgenson LA et al. 2015 The BRAIN Initiative: developing technology to catalyse neuroscience discovery. Phil Trans. R. Soc. B. Kevin L. Briggman, William B. Kristan, Jesus E. Gonzalez, David Kleinfeld, and Roger Y. Tsien. Chapter 6 - Monitoring Integrated Activity of Individual Neurons Using FRET-Based Voltage-Sensitive Dyes. Springer International Publishing Switzerland. Membrane Potential Imaging in the Nervous System and Heart, Advances in Experimental Medicine and Biology, 149-169. Roger Y. Tsien.

3

Description of C. Elegans Movie

http://tsienlab.ucsd.edu/HTML/Discussion/CoverPicture.htm

Depicted is a conceptual model of a fusion between GFP (coordinates from Protein Data Bank structure 1HUY. And a tetracysteine containing peptide (CCXXCC) that has been site-specifically labeled with the ‘ re. Airpin-binding’ dye (ReAsH). This type of construct has proven useful for characterizing the biarsenical-tetracysteine labeling system in live cells due to ability of GFP and ReAsH to undergo fluorescence resonance energy transfer (FRET) (see for example Griffin et al. (1998). Robert E. Campbell.

4

Links

http://tsienlab.ucsd.edu/Links.htm

Optical filters for biological applications. Optical filters for biological applications. Optical filters for biological applications. Filter changer and light sources for microscopes. Monochrometer-based plate reader (Safire).

5

Images

http://tsienlab.ucsd.edu/Images.htm

640 X 640 Pixels. 1024 X 1024 Pixels. 1600 X 1600 Pixels. 2900 X 2900 Pixels. 1024 X 768 Pixels. Agar Plate of Fluorescent Bacteria Colonies. 800 X 800 Pixels. Composite of Fluorescent Cells. 1375 X 1111 Pixels.

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TOTAL PAGES IN THIS WEBSITE

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LINKS TO THIS WEBSITE

gum2012.fionastoreydesign.co.uk gum2012.fionastoreydesign.co.uk

Fluorescence microscopy essentials - Jeremy Sanderson

http://gum2012.fionastoreydesign.co.uk/fluoresence.html

Tips: Collecting Good Images. Lateral and Axial Resolving Power. Collecting Data - Nyquist Sampling. Adding a Scale Bar. Basics of fluorescence bleed-through. In describing spectral overlap artifacts, the terms bleed-through. Are often used interchangeably. Bleed-through occurs when more than one fluorophore is used to stain cells or tissues. There are three categories of bleed-through. Interaction between two fluorophores' emission: cross emission. See useful tutorial on bleed-through. Excitation, dichr...

dariocolettithescientist.blogspot.com dariocolettithescientist.blogspot.com

Dario Coletti's science cellar: METHOD: INNOVATIVE RAPID PROTOCOL TO QUANTIFY NUCLEAR STAINING

http://dariocolettithescientist.blogspot.com/2013/04/method-innovative-rapid-protocol-to.html

Dario Coletti's science cellar. Through this blog I present and share my scientific interests and professional skills. RESEARCH INTERESTS: Cellular and molecular mechanisms underlying muscle wasting. There is no effective pharmacological or palliative intervention against cachexia in the clinical practice to date. Current treatments in human clinical trials aimed at cachexia include the use of growth factors, anabolic agents, appetite stimulants and nutrients, anti-inflammatory therapies and exercise.

dariocolettithescientist.blogspot.com dariocolettithescientist.blogspot.com

Dario Coletti's science cellar: METHODS: Anesthesia for rodents

http://dariocolettithescientist.blogspot.com/2013/03/anesthesia-for-rodents.html

Dario Coletti's science cellar. Through this blog I present and share my scientific interests and professional skills. RESEARCH INTERESTS: Cellular and molecular mechanisms underlying muscle wasting. There is no effective pharmacological or palliative intervention against cachexia in the clinical practice to date. Current treatments in human clinical trials aimed at cachexia include the use of growth factors, anabolic agents, appetite stimulants and nutrients, anti-inflammatory therapies and exercise.

dariocolettithescientist.blogspot.com dariocolettithescientist.blogspot.com

Dario Coletti's science cellar: METHODS: Visualisation of myosin isoforms by elecrophoresis and silver stain

http://dariocolettithescientist.blogspot.com/2012/05/methods-visualisation-of-myosin.html

Dario Coletti's science cellar. Through this blog I present and share my scientific interests and professional skills. RESEARCH INTERESTS: Cellular and molecular mechanisms underlying muscle wasting. There is no effective pharmacological or palliative intervention against cachexia in the clinical practice to date. Current treatments in human clinical trials aimed at cachexia include the use of growth factors, anabolic agents, appetite stimulants and nutrients, anti-inflammatory therapies and exercise.

dariocolettithescientist.blogspot.com dariocolettithescientist.blogspot.com

Dario Coletti's science cellar: POSTDOC POSITION IN SKELETAL MUSCLE PHYSIO-PATHOLOGY AVAILABLE IMMEDIATELY

http://dariocolettithescientist.blogspot.com/2014/07/postdoc-position-in-skeletal-muscle.html

Dario Coletti's science cellar. Through this blog I present and share my scientific interests and professional skills. RESEARCH INTERESTS: Cellular and molecular mechanisms underlying muscle wasting. There is no effective pharmacological or palliative intervention against cachexia in the clinical practice to date. Current treatments in human clinical trials aimed at cachexia include the use of growth factors, anabolic agents, appetite stimulants and nutrients, anti-inflammatory therapies and exercise.

dariocolettithescientist.blogspot.com dariocolettithescientist.blogspot.com

Dario Coletti's science cellar: METHODS: 4 color IF for extra-cellular matrix and myosin isoforms

http://dariocolettithescientist.blogspot.com/2012/12/lab-methods-4-color-if-for-extra.html

Dario Coletti's science cellar. Through this blog I present and share my scientific interests and professional skills. RESEARCH INTERESTS: Cellular and molecular mechanisms underlying muscle wasting. There is no effective pharmacological or palliative intervention against cachexia in the clinical practice to date. Current treatments in human clinical trials aimed at cachexia include the use of growth factors, anabolic agents, appetite stimulants and nutrients, anti-inflammatory therapies and exercise.

dariocolettithescientist.blogspot.com dariocolettithescientist.blogspot.com

Dario Coletti's science cellar: Grip stenght test

http://dariocolettithescientist.blogspot.com/2012/03/grip-stenght-test.html

Dario Coletti's science cellar. Through this blog I present and share my scientific interests and professional skills. RESEARCH INTERESTS: Cellular and molecular mechanisms underlying muscle wasting. There is no effective pharmacological or palliative intervention against cachexia in the clinical practice to date. Current treatments in human clinical trials aimed at cachexia include the use of growth factors, anabolic agents, appetite stimulants and nutrients, anti-inflammatory therapies and exercise.

dariocolettithescientist.blogspot.com dariocolettithescientist.blogspot.com

Dario Coletti's science cellar: METHODS: Phosphate Buffered Solutions in our lab

http://dariocolettithescientist.blogspot.com/2013/03/methods-phosphate-buffered-solutions-in.html

Dario Coletti's science cellar. Through this blog I present and share my scientific interests and professional skills. RESEARCH INTERESTS: Cellular and molecular mechanisms underlying muscle wasting. There is no effective pharmacological or palliative intervention against cachexia in the clinical practice to date. Current treatments in human clinical trials aimed at cachexia include the use of growth factors, anabolic agents, appetite stimulants and nutrients, anti-inflammatory therapies and exercise.

dariocolettithescientist.blogspot.com dariocolettithescientist.blogspot.com

Dario Coletti's science cellar: METHODS: Murine muscle dissection from the hinlimb

http://dariocolettithescientist.blogspot.com/2012/05/methods-murine-muscle-dissection-from.html

Dario Coletti's science cellar. Through this blog I present and share my scientific interests and professional skills. RESEARCH INTERESTS: Cellular and molecular mechanisms underlying muscle wasting. There is no effective pharmacological or palliative intervention against cachexia in the clinical practice to date. Current treatments in human clinical trials aimed at cachexia include the use of growth factors, anabolic agents, appetite stimulants and nutrients, anti-inflammatory therapies and exercise.

dariocolettithescientist.blogspot.com dariocolettithescientist.blogspot.com

Dario Coletti's science cellar: Indo-Italian Forum on Biomaterials and Tissue Engineering

http://dariocolettithescientist.blogspot.com/2012/03/indo-italian-forum-on-biomaterials-and.html

Dario Coletti's science cellar. Through this blog I present and share my scientific interests and professional skills. RESEARCH INTERESTS: Cellular and molecular mechanisms underlying muscle wasting. There is no effective pharmacological or palliative intervention against cachexia in the clinical practice to date. Current treatments in human clinical trials aimed at cachexia include the use of growth factors, anabolic agents, appetite stimulants and nutrients, anti-inflammatory therapies and exercise.

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